Preparing and Maintaining a Sterile Field

Key Points

  • Sterile technique requires continuous contamination prevention from setup through procedure completion.
  • Choose field intensity before setup: general aseptic field for easily protected key parts/sites versus critical aseptic field when protection is difficult and full sterile-field control is required.
  • Hand hygiene, clean dry surfaces, and in-date intact supplies are required before opening sterile items.
  • The 1 in (2.5 cm) package border is considered unsterile and must not be used as a sterile working area.
  • A sterile field is considered contaminated when unattended, out of view, below waist level, above shoulder level, below table level, wet, or near holes/tears.

Sterile barrier attire and sterile field setup example showing gowning and instrument preparation Illustration reference: OpenStax Fundamentals of Nursing Ch.10.2.

Equipment

  • Sterile package or kit with intact seal and valid expiration date
  • Clean, dry work surface
  • Sterile gloves (with backup pair available)
  • Sterile bowl/tray and sterile solution when needed

Procedure Steps

  1. Perform hand hygiene before beginning any sterile setup activity.
  2. Identify key parts and key sites for the planned procedure and determine whether a general aseptic field or critical aseptic field is required.
  3. Prepare a clean, dry workspace and gather all needed supplies within reach.
  4. Verify sterile package integrity before opening: no moisture exposure, holes, tears, or failed sterilization indicator; confirm expiration date.
  5. Open sterile kit flaps in sequence: first flap away from body, then side flaps one at a time, then final flap toward body, while touching only flap edges.
  6. While opening kits/wrappers, avoid positioning arms/body directly above sterile field and prevent opened flaps from springing back over sterile contents.
  7. Don sterile gloves using cuff-touch-only method while keeping hands above waist and in visual field.
  8. Maintain direct line of sight to the sterile field at all times; if field is not visible, consider sterility broken.
  9. Avoid reaching over field and prevent nonsterile sleeves, equipment, or dangling objects from entering field space.
  10. Keep all sterile items above waist level; items lowered below waist are nonsterile.
  11. Treat these zones/states as contaminated: 1 in field edge, drape below tabletop plane, field above shoulder level, unattended/out-of-view field, and any area near tears/moisture wick-through.
  12. Handle key parts only when needed and use non-touch technique whenever possible.
  13. Sequence setup and procedure steps from clean to dirty to reduce contamination transfer.
  14. When dispensing sterile items, prevent contact with outer packaging/former package seal; for heavy or irregular items, use sterile-gloved transfer assistance when needed.
  15. Open peel pouches by pulling the sealed edges apart and drop/dispense items from about 6 in (15 cm) above the field without crossing over the field.
  16. If using sterile solution, pour from the side into sterile container from about 6 in (15 cm) away, avoid splashing, and do not restart pouring from the same container edge once stopped.
  17. Don sterile gloves away from the sterile field to reduce field contamination risk.
  18. Replace any wet or contaminated items immediately and re-establish sterility before proceeding.

Common Errors

  • Touching or using the 1 in package border as sterile immediate field contamination.
  • Turning away from field sterility cannot be assured and field integrity is lost.
  • Allowing sterile items below waist or wetting field conversion to nonsterile status.
  • Pouring directly over field or too quickly splash contamination risk.
  • Restarting a sterile-solution pour after stopping container edge contamination transfer risk.